Lineage 1

Percentage of genes called significant as we change q-value threshold

## 62.2% of the genes are called significant when we require the adjusted p-value to be smaller than 0.01

Heatmap of most significant genes

Visualization of 9 most significant genes

Visualization of 9 least significant genes

Lineage 2

Percentage of genes called significant as we change q-value threshold

## 76.16% of the genes are called significant when we require the adjusted p-value to be smaller than 0.01

Heatmap of most significant genes

Visualization of 9 most significant genes

Visualization of 9 least significant genes

Observation

LineagePulse works well in detecting time-varying genes. However, it is very sensitive to outliers as several of the most significant genes are detected due to outliers (?).